Ferrozine, chemically 3-(2-Pyridyl)-5,6-bis(4-phenylsulfonic acid)-1,2,4-triazine disodium salt, is a water-soluble solid. It is complexometric reagent for detection and estimation of iron.

It is synthesized by condensation of 2,6-dimethyl pyridine with 3,5-dinitrobenzenesulfonic acid, followed by reduction with Fe powder, and then sulfonation with fuming sulfuric acid. Purification occurs via crystallization from water.

acid sodium salt
CAS no.28048-33-1
EINECS no.248-797-6
Molecular formulaC20H12-13N4Na1-2O6S2·xH2O
Molecular weight468.47 (anion)
Determine the iron content in food, water, chemicals, and biological samples
Study the metabolism of iron in cells and tissues
Monitor the environmental pollution caused by heavy metals
Preparation of iron complexes for catalysis
Synthesis of iron nanoparticles for biomedicine
Develop new sensors, biomaterials, and drug delivery systems
Improve efficacy and safety of existing medicinal treatments
Numerous other applications in scientific experiments, especially in the fields of analytical chemistry, biochemistry, and environmental science


AppearanceGreenish yellow powder
Solubility (C5%; w/v. in acetate buffer, pH 5.5)Yellow, clear
Identification by UV (c = 1%, w/v, d = 1 cm)Should correspond to standard
Max 282-284 nm
Specific Extinction at absorption Max 385-465
Identification by IRShould correspond to standard
Sodium by flame photometry, based on hydrous substance%4.0 – 9.0
KF Water%≤10.0
Assay Ferrozine anion (Photometric), based on hydrous substance%90.0 – 110.0

Stored at ambient temperature.

25 kg drum.

REACH Status
Not registered yet.

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