INTRODUCTION
FerroQuant™ Pro is a water-soluble chromogenic reagent widely used for the spectrophotometric determination of iron. Chemically, it is a ferroin-type ligand that forms a stable, intensely colored complex with ferrous ions (Fe²⁺).
MANUFACTURE
Ferrozine is synthesized through a multistep process starting from 2-cyanopyridine. The synthesis involves hydrazine treatment, followed by reaction with benzil to form a triazine intermediate, which is subsequently sulfonated and converted to its sodium salt to yield Ferrozine. All steps are performed under controlled conditions to ensure consistent product quality.
| Synonyms | Benzenesulfonic acid, 4,4′-[3-(2-pyridinyl)-1,2,4-triazine-5,6-diyl]bis-, disodium salt; Ferrospectral; Ferrozine Disodium Salt |
| CAS no. | 28048-33-1 |
| EINECS no. | 248-797-6 |
| Molecular formula | C20H12N4Na2O6S2 |
| Molecular weight | 514.44 |
| Structure |  |
APPLICATIONS
FerroQuant™ Pro has numerous applications in analytical chemistry, biochemistry, environmental analysis and industrial testing.
| Determination of iron: |
| • It is one of the widely used colorimetric reagents for measuring Fe²⁺ and total iron. |
| Biochemical assays & Environmental analysis: |
| • Used to determine iron in biological samples such as blood, serum, cells and tissue extracts. |
| • Used for detecting iron in water, wastewater and environmental samples. |
| Food and pharmaceutical analysis: |
| • Can be used for determining iron concentrations in food products, nutritional preparations and pharmaceutical samples. |
| Iron-binding studies: |
| • Useful for investigating iron availability, iron release and iron-binding processes in biochemical systems. |
| Redox studies: |
| • Since the reagent responds specifically to Fe²⁺, it can be incorporated into assays involving the reduction of Fe³⁺ to Fe²⁺. |
| Quantitative spectrophotometry: |
| • Its strong color response provides a sensitive method for determining relatively small concentrations of iron. |
SPECIFICATIONS
| Test | Unit | Specification |
|---|---|---|
| Appearance | – | Greenish yellow powder |
| Solubility (C5%; w/v. in acetate buffer, pH 5.5) | – | Yellow, Clear |
| Identification by UV (c = 1%, w/v, d = 1 cm) | – | Should correspond to standard Max 282-284 nm Specific extinction at absorption Max 385-465 |
| Identification by IR | – | Should correspond to standard |
| Sodium by flame photometry, based on hydrous substance | % | 4.0 – 9.0 |
| Water Content (By KF) | % | ≤10.0 |
| Assay Ferrozine anion (Photometric), based on hydrous substance | % | 90.0 – 110.0 |
| Iron (Fe) content (By ICP-MS) | ppm | NMT 10 |
| Mercury content (By ICP-MS) | – | Should be absent |
| Purity by HPLC | % | To report |
| Purity by LCMS | % | To report |
STORAGE
Keep in dry, cool place in tightly closed containers between +15 °C – +40 °C.
PACKING
25 kg HDPE drum
REACH Status
Not registered
ExSyn offers FerroQuant™ Pro on commercial scales and welcomes enquiries. No matter the quantity you need, our exceptional quality and service will make ExSyn your supplier of choice! If you need any additional information or SDS, please contact us.
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